NOTCH1 Rabbit Polyclonal Antibody

CAT#: TA319200

Rabbit polyclonal anti-NOTCH 1 antibody

Product Images

Anti-Human NOTCH 1 (Cleaved N Terminal) was used at 1:500 to detect mouse Notch 1. Lane M: Marker. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular)-myc. Lane 4: N2 (mouse deleted extracellular)-myc. Lane 5: N2 (mouse intracellular)-myc. Lane 6: N3 (mouse deleted extracellular)-myc. Lane 7: N3 (mouse intracellular)-myc. Lane 8: N4 (mouse deleted extracellular)-myc. Lane 9: N4 (mouse intracellular)-myc. Lane 10: N1 (mouse deleted extracellular) (V to G)-myc.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:20,000 - 1:60,000, WB: 1:500- 1:2,000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: notch 1
• Database Link:
  NP_060087
  Entrez Gene 18128 MouseEntrez Gene 25496 RatEntrez Gene 4851 Human
  UniProt ID P46531
• Synonyms: AOS5; AOVD1; hN1; TAN1
• Note: Anti Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway
• Protein Pathways: Dorso-ventral axis formation, Notch signaling pathway, Prion diseases