GSK3 alpha (GSK3A) Rabbit Polyclonal Antibody

CAT#: TA319231

Rabbit polyclonal GSK3 Alpha phospho S21 antibody

Product Images

WB using Anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A in various human derived 293T cell extracts. Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM [LY294002] (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min. Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (Lane 2) and diminished when an inhibitor is added (lane 3). Primary antibody was used at 1:1,000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:3,000, IF: User Optimized
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 16-25 of human GSK3 alpha.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glycogen synthase kinase 3 alpha
• Database Link:
  NP_063937
  Entrez Gene 2931 Human
  UniProt ID P49840
• Synonyms: DKFZp686D0638
• Note: Glycogen synthase kinase 3 alpha (GSK3A) belongs to the ser/thr family of protein kinases, Cdc2/cdkx subfamily; gsk-3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c-jun. GSK3A is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A) and beta (GSK3B), show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway