NEK2 Rabbit Polyclonal Antibody

CAT#: TA319262

Rabbit polyclonal anti-NEK2 antibody

Product Images

Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000, IF: User Optimized
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 287-299 of Human NEK2 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NIMA related kinase 2
• Database Link:
  NP_001191111
  Entrez Gene 4751 Human
  UniProt ID P51955
• Synonyms: HsPK21; NEK2A; NLK1; PPP1R111; RP67
• Note: NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G₂-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G₂. This expression pattern is highly reminiscent of that of A and B cyclins.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase