TGF beta 1 (TGFB1) Rabbit Polyclonal Antibody

CAT#: TA319281

Rabbit polyclonal TGF beta 1 antibody

Product Images

WB analysis using Anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at RT for 1 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG (H&L) diluted 1:2, 500.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:40,000, WB: 1:100 - 1:500, IF: User Optimized
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TGF beta 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transforming growth factor beta 1
• Database Link:
  NP_000651
  Entrez Gene 7040 Human
  UniProt ID P01137
• Synonyms: CED; DPD1; LAP; TGFB; TGFbeta
• Note: TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, including three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule; the amino acid sequence between human and mouse differs by only one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues, such as placenta and kidneys.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, TGF-beta signaling pathway