PTEN Rabbit Polyclonal Antibody

CAT#: TA319293

Rabbit polyclonal anti-PTEN-P1 antibody

Product Images

WB using Anti-PTEN-P1 antibody shows detection of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa (lane 4), HEK293 (lane 5) and MCF7 (lane 6). The band at ~55 kDa (arrowhead) corresponds to PTEN-P1. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. Primary antibody was used at 1:500. IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX was used at 1:10000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:160,000, WB: 1:500 to 1:2,000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminal end of human PTEN-P1 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: phosphatase and tensin homolog
• Database Link:
  NP_000305
  Entrez Gene 5728 Human
  UniProt ID P60484
• Synonyms: 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1
• Note: This gene (PTENP1) is a highly homologous pseudogene of PTEN. PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. PTEN is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tension like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction