CDC27 Rabbit Polyclonal Antibody

CAT#: TA319297

Rabbit polyclonal CDC27 phospho T244 antibody

Product Images

WB using Anti-CDC27 pT244 antibody shows detection of a band ~92 kDa corresponding to phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Phosphorylated CDC27 is mostly present only in cell preparations arrested in mitosis.The primary antibody was diluted to 1:500. HRP Gt-a-Rabbit IgG [H&L] MX was used at 1:5000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:8,000 - 1:30,000, WB: 1:300 - 1:2,000, IP: 1:100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 239-249 of Human CDC27.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: cell division cycle 27
• Database Link:
  NP_001107563
  Entrez Gene 996 Human
  UniProt ID P30260
• Synonyms: ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
• Note: Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis