KIAA0652 (ATG13) Rabbit Polyclonal Antibody

CAT#: TA319365

Rabbit polyclonal anti-ATG13 antibody

Product Images

WB using Anti-ATG13 antibody shows detection of ATG13 in 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13), right lane, but not in the left lane in which was loaded kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13. Detection is demonstrated at approximately 57 kDa. primary antibody was used at 1ug/mL overnight at 4°C. After washing, the membrane was probed with Goat Anti-Rabbit HRP secondary 1:5000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:220,000-1:250,000, WB: 1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the S318 region of ATG13.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: autophagy related 13
• Database Link:
  NP_001136145
  Entrez Gene 9776 Human
  UniProt ID O75143
• Synonyms: KIAA0652; PARATARG8
• Note: ATG13 is a target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1 through their stable complex, and the regulation of ATG13-ULK1-RB1CC1. ATG13 also forms a stable complex with FIP200. Ulk1 phosphorylates ATG13 on S318 and promotes its release to damaged mitochondria. Autophagy is a normal process in eukaryotes required for turnover of cellular components during starvation and stress. It plays an essential role in cellular differentiation, cell death and aging. Defects in this evolutionarily conserved process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. ATG13 is one of several ATG genes required for autophagosome formation in mammalian cells. mTOR interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1.