ATR Rabbit Polyclonal Antibody

CAT#: TA319394

Rabbit polyclonal anti-ATR antibody

Product Images

Western blot using anti-ATR antibody shows detection of ATR in HeLa cell nuclear extract (lane 1). Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ?g peptide for 1 h at room temperature followed by centrifugation). A 4-8% gradient gel was used for separation. Goat serum was used at 5% for blocking. The arrowhead corresponds to 301 kDa ATR when compared to MW markers (Lane M). The primary antibody was used at a 1:1, 400 dilution.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:15,000 - 1:70,000, WB: 1:1,000 - 1:5,000
• Reactivities: Fish, Human, Monkey, Mouse, Rat, Xenopus, Dog
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ATR protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATR serine/threonine kinase
• Database Link:
  NP_001175
  Entrez Gene 245000 MouseEntrez Gene 685055 RatEntrez Gene 100856315 DogEntrez Gene 714443 MonkeyEntrez Gene 545 Human
  UniProt ID Q13535
• Synonyms: FCTCS; FRP1; MEC1; SCKL; SCKL1
• Note: Ataxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidyl-inositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR activates checkpoint signaling upon genotoxic stresses, such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. ATR is a member of the DNA-PK kinase family and is closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes, including histone deacetylases (HDAC1, HDAC2 and CHD4). ATR is known to phosphorylate BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. ATR is a nuclear protein, but can also be found in PML nuclear bodies in certain cell types. ATR is recruited to chromatin during S-phase and redistributes to discrete nuclear foci upon DNA damage, hypoxia or replication fork stalling.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, p53 signaling pathway