alpha Adaptin (AP2A2) Goat Polyclonal Antibody

CAT#: TA319408

Goat polyclonal anti-AP2A antibody

Product Images

WB using Anti-AP2A antibody shows detection of a band just below 100 kDa correspond-ing to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effect-ively blocks the specific reactivity of this antibody with AP2A. Primary antibody was used at 1:500.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000, IP: 1:100
• Reactivities: Chimpanzee, Chicken, Human, Mouse, Orang-Utan, Rat, Dog
• Host: Goat
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adaptor related protein complex 2 alpha 2 subunit
• Database Link:
  NP_036437
  Entrez Gene 11772 MouseEntrez Gene 81637 RatEntrez Gene 475994 DogEntrez Gene 161 Human
  UniProt ID O94973
• Synonyms: ADTAB; CLAPA2; HIP-9; HIP9; HYPJ
• Note: The AP2A1 and AP2A2 represent the AP2 alpha proteins that are found in the AP2 complex in clathrin-coated vesicles. AP2A is also known as Alpha-adaptin A, Adaptor protein complex AP-2 alpha-1 subunit, Clathrin assembly protein complex 2 alpha-A large chain, 100 kDa coated vesicle protein A and Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit. The AP2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles, which link clathrin to receptors in vesicles.

Reference Data

• Protein Pathways: Endocytosis, Huntington's disease