GPC1 Rabbit Polyclonal Antibody

CAT#: TA319424

Rabbit polyclonal Glypican-1 antibody

Product Images

WB of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4°C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band (s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:10,000 - 1:50,000, WB: 1:500- 1:2,000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-Glypican-1 protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glypican 1
• Database Link:
  NP_002072
  Entrez Gene 14733 MouseEntrez Gene 58920 RatEntrez Gene 2817 Human
  UniProt ID P35052
• Synonyms: glypican
• Note: Glypican-1 antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.

Reference Data

• Protein Families: Druggable Genome