DNA Polymerase beta (POLB) Rabbit Polyclonal Antibody

CAT#: TA319428

Rabbit polyclonal anti-POLB antibody

Product Images

Western Blot of Rabbit Anti-POLß (DNA polymerase beta) Antibody (TA319428). Lane 1: LN428 FLAG POLß (control). Lane 2: A172. Load: 35 Âug per lane. Primary antibody: POLß antibody at 1:2000 for overnight at 4°C. Secondary antibody: IRDye800â„¢ goat anti-rabbit at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band (s): unknown band ~75 kDd.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:50,000, WB: 0.5 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptidecorresponding to a region near the C-terminus of the POLβ protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: polymerase (DNA) beta
• Database Link:
  NP_002681
  Entrez Gene 5423 Human
  UniProt ID P06746
• Synonyms: MGC125976
• Note: The POLβ protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair