MAD2L2 Rabbit Polyclonal Antibody

CAT#: TA319443

Rabbit polyclonal anti-MAD2L2 antibody

Product Images

Rabbit anti-MAD2L2 was used at 1:500to detect human MAD2L2 by WB. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa).

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  Affinity Purified anti-MAD2L2 antibody shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:7,500 - 1:30,000, WB: 1:500 - 1:2,000, IHC: 1:500 - 1:2,000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2. MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: MAD2 mitotic arrest deficient-like 2 (yeast)
• Database Link:
  NP_006332
  Entrez Gene 10459 Human
  UniProt ID Q9UI95
• Synonyms: MAD2B; POLZ2; REV7
• Note: MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation