Mdm2 Rabbit Polyclonal Antibody

CAT#: TA319466

Rabbit polyclonal anti-MDM2 antibody

Product Images

WB using Anti-MDM2 (Rabbit) to detect a band (arrow) corresponding to mouse MDM2 protein present in mouse MEF cells (lane 2) but not human kidney HEK293 cells (lane 1). The primary antibody was diluted to 1:500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:3,000 - 1:12,000, WB: 1:500 - 1:2,000, IP: 1:100
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 177-195 of mouse MDM2.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: transformed mouse 3T3 cell double minute 2
• Database Link:
  NP_034916
  Entrez Gene 17246 Mouse
  UniProt ID P23804
• Synonyms: Hdm2; HDMX; MGC5370; MGC71221; OTTHUMP00000183495
• Note: MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53-mediated transactivation. MDM2 binds to p53 and amplification of MDM2 in sarcomas leads to escape from p53-regulated growth control. This mechanism of tumorigenesis parallels that for virus-induced tumors in which viral oncogene products bind to and functionally inactivate p53. Over-expression of the