Sts1 (UBASH3B) Rabbit Polyclonal Antibody

CAT#: TA319507

Rabbit polyclonal anti-Sts-1 antibody

Product Images

WB using Anti-Sts-1 antibody shows detection of a band ~70 kDa corresponding to mouse Sts-1. Approximately 1.0 ?g of recombinant (truncated) Sts-1-GST was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 0.3?ug/ml overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:3,000, IF: User Optimized
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of mouse Sts-1.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ubiquitin associated and SH3 domain containing B
• Database Link:
  NP_116262
  Entrez Gene 72828 Mouse
  UniProt ID Q8TF42
• Synonyms: p70; STS-1; STS1; TULA-2; TULA2
• Note: Sts-1 is a protein that inhibits endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. Sts-1 and Sts-2 (formerly p70 and Clip4, respectively) have been found to interact with Cbl, an ubiquitin ligase that plays a critical role in attenuation of receptor tyrosine kinase signaling by inducing ubiquitination and promoting their sorting for endosomal degradation. Sts-1 and Sts-2 contain SH3 domains that interact with Cbl, Ub-associated domains, which bind directly to mono-Ub or to the EGFR/Ub chimera, as well as phosphoglycerate mutase domains that mediate oligomerization of Sts-1/2. Sts-1 and Sts-2 also have been found to negatively regulate signaling pathways that control T cell receptors, which in turn affect the extent and duration of the T cell response to foreign pathogens.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS