RAD52 Rabbit Polyclonal Antibody

CAT#: TA319514

Rabbit polyclonal anti-RAD52 antibody

Product Images

WB analysis using Anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360 - 375 of the Human Rad 52 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAD52 homolog, DNA repair protein
• Database Link:
  NP_602296
  Entrez Gene 5893 Human
  UniProt ID P43351
• Synonyms: RP11-359B12.2
• Note: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination