PDCD4 Mouse Monoclonal Antibody [Clone ID: 9G6]

CAT#: TA319575

Mouse monoclonal Pdcd4 phospho S457 antibody (Phospho-specific)

Product Images

Western Blot of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Lane 1: Pdcd4 recombinant protein. Lane 2: none. Load: 100 ng per lane. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Pdcd4 phospho S457. Other band (s): Pdcd4 phospho S457 splice variants and isoforms

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  ELISA of Mouse anti-Pdcd4 phospho S457 Biotin Conjugated antibody. Antigen: BSA conjugates of Pdcd4 phospho S457 and Pdcd4 non-phospho S457. Coating amount: 0.1 ug per well. Primary antibody: Pdcd4 phospho S457 Biotin Conjugated antibody at 5 ug/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Pdcd4 phospho S457 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100)

Specifications

Product Data

• Clone Name: 9G6
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IP: 1:1,000 - 1:5,000
• Reactivities: Human, Mouse, Rat, Xenopus
• Modifications: Phospho-specific
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Database Link:
  NP_663314
  Entrez Gene 18569 MouseEntrez Gene 64031 RatEntrez Gene 27250 Human
  UniProt ID Q53EL6
• Synonyms: H731
• Note: Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Reference Data

• Protein Families: Druggable Genome