DPAGT1 Rabbit Polyclonal Antibody

CAT#: TA319635

Rabbit Polyclonal DPAGT1 Antibody

Product Images

Western blot analysis of DPAGT1 in mouse kidney tissue lysate with DPAGT1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of DPAGT1 in human kidney tissue with DPAGT1 antibody at 2.5 ug/mL.

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  Immunofluorescence of DPAGT1 in human kidney tissue with DPAGT1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 2.5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DPAGT1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human DPAGT1.
• Formulation: DPAGT1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: DPAGT1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
• Database Link:
  NP_001373
  Entrez Gene 13478 MouseEntrez Gene 1798 Human
  UniProt ID Q9H3H5
• Background: DPAGT1 Antibody: The UDP-N-acetylglucosamine-dolichyl-phosphate N-acetyl-glucosaminephosphotransferase (DPAGT1) is an enzyme that catalyzes the first step in the dolichol-linked oligosaccharide pathway for glycoprotein biosynthesis. Mutations in this integral endoplasmic reticulum (ER) membrane protein enzyme belongs to the glycosyltransferase family 4 results in the congenital disorder of glycosylation type Ij with symptoms such as severe hypotonia, medically intractable seizures, mental retardation, microcephaly, and exotropia. Recent experiments have shown that DPAGT1 is a target of the Wnt/beta-catenin signaling pathway, with Wnt3a inducing higher DPAGT1 mRNA expression.
• Synonyms: ALG7; CDG-Ij; CDG1J; CMSTA2; D11S366; DGPT; DPAGT; DPAGT2; G1PT; GPT; UAGT; UGAT

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis