Endo G (ENDOG) Mouse Monoclonal Antibody

CAT#: TA319641

Mouse EndoG Monoclonal Antibody

Product Images

Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 2.5 and (B) 5 ug/mL.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 2.5 - 5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein corresponding to amino acids 51 - 140 of human EndoG.
• Formulation: EndoG Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: EndoG Monoclonal Antibody is immunoaffinity chromotography purified IgG.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: endonuclease G
• Database Link:
  NP_004426
  Entrez Gene 13804 MouseEntrez Gene 362100 RatEntrez Gene 2021 Human
  UniProt ID Q14249
• Background: EndoG Monoclonal Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
• Synonyms: FLJ27463

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis