PHLPP (PHLPP1) Rabbit Polyclonal Antibody

CAT#: TA319850

Rabbit Polyclonal PHLPP1 Antibody

Product Images

Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 antibody at 2.5 ug/mL.

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  Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 antibody at 5 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 2 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1.
• Formulation: PHLPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: PHLPP1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 189 kDa
• Gene Name: PH domain and leucine rich repeat protein phosphatase 1
• Database Link:
  NP_919431
  Entrez Gene 23239 Human
  UniProt ID O60346
• Background: PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
• Synonyms: PHLPP; PLEKHE1; PPM3A; SCOP