ANAPC13 Rabbit Polyclonal Antibody

CAT#: TA319874

Rabbit Polyclonal APC13 Antibody

Product Images

Western blot analysis of APC13 in Jurkat cell tissue lysate with APC13 antibody at 1ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Western blot analysis with 25ng of recombinant APC13 protein with APC13 antibody at 1ug/ml.

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  Immunofluorescence of APC13 in Jurkat cells with APC13 antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: APC13 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13. The immunogen is located within the last 50 amino acids of APC13.
• Formulation: APC13 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: APC13 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: anaphase promoting complex subunit 13
• Database Link:
  NP_056206
  Entrez Gene 69010 MouseEntrez Gene 685029 RatEntrez Gene 25847 Human
  UniProt ID Q9BS18
• Background: APC13 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.
• Synonyms: APC13; SWM1

Reference Data

• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis