Apc5 (ANAPC5) Rabbit Polyclonal Antibody

CAT#: TA319925

Rabbit Polyclonal APC5 Antibody

Product Images

Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 ug/mL.

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  Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5.
• Formulation: APC5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: APC5 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: anaphase promoting complex subunit 5
• Database Link:
  NP_057321
  Entrez Gene 59008 MouseEntrez Gene 288671 RatEntrez Gene 51433 Human
  UniProt ID Q9UJX4
• Background: APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.
• Synonyms: APC5

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis