DDA1 Rabbit Polyclonal Antibody

CAT#: TA320007

Rabbit Polyclonal DDA Antibody

Product Images

Western blot analysis of DDA1 in mouse heart tissue lysate with DDA1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide

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  Immunohistochemistry of DDA1 in mouse heart tissue with DDA1 antibody at 5 ug/mL.

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  Immunofluorescence of DDA1 in mouse heart tissue with DDA1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 ug/mL, ICC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DDA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DDA1.
• Formulation: DDA Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: DDA Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: DET1 and DDB1 associated 1
• Database Link:
  NP_076955
  Entrez Gene 66498 MouseEntrez Gene 688813 RatEntrez Gene 79016 Human
  UniProt ID Q9BW61
• Background: DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.
• Synonyms: C19orf58; PCIA1