ABH2 (ALKBH2) Rabbit Polyclonal Antibody

CAT#: TA320088

Rabbit Polyclonal ALKBH2 Antibody

Product Images

Western blot analysis of ALKBH2 in human kidney tissue lysate with ALKBH2 antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of ALKBH2 in human kidney tissue with ALKBH2 antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, ICC: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ALKBH2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ALKBH2.
• Formulation: ALKBH2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: ALKBH2 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase
• Database Link:
  NP_001001655
  Entrez Gene 231642 MouseEntrez Gene 304578 RatEntrez Gene 121642 Human
  UniProt ID Q6NS38
• Background: ALKBH2 Antibody: The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA; ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.
• Synonyms: ABH2

Reference Data

• Protein Families: Druggable Genome