Cd160 Rat Monoclonal Antibody [Clone ID: eBioCNX46-3 (CNX46-3)]

CAT#: TA320237

Rat Anti-Mouse CD160 Purified (50 ug)

Product Images

C57BL/6 splenocytes were stained with 0.5 ug of Anti-Mouse CD160 Purified followed by F (ab')2 Anti-Rat IgG PE and subsequently with Anti-Mouse CD3e FITC and Anti-Mouse NK1.1 APC demonstrates staining of Anti-Mouse CD160 Purified on NK1.1+CD3- cells (red histogram and NK1.1+CD3+ cells (green histogram), as gated in the dot plot (left).

Specifications

Product Data

• Clone Name: eBioCNX46-3 (CNX46-3)
• Applications: FC
• Recommended Dilution: Flow, Functional Assay, IP, WB
• Reactivities: Mouse
• Host: Rat
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD160 antigen
• Database Link:
  NP_061237
  Entrez Gene 54215 Mouse
  UniProt ID O88875
• Background: CD160 is a glycosylphosphatidylinositol (GPI)-anchored Ig-like glycoprotein first identified on human lymphocytes with the monoclonal antibody BY55. In mice, CD160 is expressed on almost all (intestinal intraepithelial lymphocytes) iIELs, NKT cells, most TCRγδ T cells, few NK cells and a minor subset of CD8+ T cells. Murine CD160 has been shown to bind to a wide range of classical and non classical MHC class I molecules and regulate NK cell activation. In vitro, CD3 activation of murine CD8+ T cells increases the expression of CD160 and induces the release of soluble CD160 (sCD160). In human, CD160 mAb cross-linking triggers TNF alpha, IFN gamma and IL-6 cytokine production by peripheral blood NK cells and inhibits tube formation and induces apoptosis of endothelial cells. In mice, cross-linking of CD160 with the CNX46-3 antibody regulates NK cell activation both positively and negatively, depending on the stimulus.
• Synonyms: BY55; FLJ46513; NK1; NK28; OTTHUMP00000015585