RP105 (CD180) Mouse Monoclonal Antibody [Clone ID: MHR73-11]

CAT#: TA320239

Mouse Anti-Human CD180 (RP105) Purified (25 ug)

Product Images

Staining of normal human peripheral blood cells with 1 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 1 ug of Anti-Human CD180 (RP105) Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the lymphocyte gate were used for analysis.

Specifications

Product Data

• Clone Name: MHR73-11
• Applications: FC
• Recommended Dilution: Flow, IHC, Functional Assay, IP
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD180 molecule
• Database Link:
  NP_005573
  Entrez Gene 4064 Human
  UniProt ID Q99467
• Background: The MHR73-11 monoclonal antibody reacts with human CD180 (RP105). This 105 kDa type I transmembrane molecule is a member of the TLR family of proteins characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. RP105 physically associates with another molecule called MD-1 and is expressed on B, monocytes/macrophages, and dendritic cells. Histological studies show that RP105 is expressed mainly on mature B cells in mantle zones, while germinal center cells are either dull or negative. The RP105/MD-1 complex in concert with TLR4 mediates B cell recognition and signaling of LPS. MHR73-11 activates B cells, leading to increases in cell size, expression of the costimulatory molecule CD80, and DNA synthesis. Moreover, ligation of RP105 protects B cells from irradiation- or dexamethasone-induced apoptosis. Thus, RP105 is a signal transduction molecule and plays a role in regulation of B cell growth and death. A significant proportion of circulating B cells in SLE patients is RP105 negative. Loss of RP105 is associated with B cell activation and increased disease activity in SLE patients.
• Synonyms: LY64; Ly78; RP105

Reference Data

• Protein Families: Druggable Genome, Transmembrane