Cd86 Rat Monoclonal Antibody [Clone ID: PO3.1]

CAT#: TA320298

Rat Anti-Mouse CD86 (B7-2) Purified (100 ug)

Product Images

Staining of 2-day unstimulated (left) or LPS-stimulated (right) C57BL/6 splenocytes with 0.25 ug of Rat IgG2b K Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD86 (B7-2) Purified (filled histogram) followed by Anti-Rat IgG FITC. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: PO3.1
• Applications: FC
• Recommended Dilution: Flow, Functional Assay
• Reactivities: Mouse
• Host: Rat
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD86 antigen
• Database Link:
  NP_062261
  Entrez Gene 12524 Mouse
  UniProt ID P42082
• Background: The PO3.1 monoclonal antibody reacts with mouse CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low level on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. CD86 is also expressed by activated mouse T cells and thioglycolate-elicited peritoneal cells. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.
• Synonyms: B7-2; B70; BU63; CD28LG2; FUN-1; LAB72; MGC34413