ICOS Mouse Monoclonal Antibody [Clone ID: ISA-3]

CAT#: TA320353

Mouse Anti-Human CD278 (ICOS) Purified (25 ug)

Product Images

Staining of CD3 + CD28 stimulated human PBMC with Anti-Human CD278 (ICOS) FITC (left), and Anti-Human CD278 (ICOS) PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis

Specifications

Product Data

• Clone Name: ISA-3
• Applications: FC
• Recommended Dilution: Flow, IHC, Functional Assay, IP
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: inducible T-cell costimulator
• Database Link:
  NP_036224
  Entrez Gene 29851 Human
  UniProt ID Q9Y6W8
• Background: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948 or 12-9948 rather than 11-9948.
• Synonyms: AILIM; CD278; CVID1

Reference Data

• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Primary immunodeficiency, T cell receptor signaling pathway