Itga5 Hamster Monoclonal Antibody [Clone ID: eBioHMa5-1 (HMa5-1)]

CAT#: TA320378

Hamster Anti-Mouse/Rat CD49e (Integrin alpha 5) Purified (50 ug)

Product Images

Staining of C57Bl/6 bone marrow cells with 0.5 ug of Armenian Hamster IgG Isotype Control Purified (left) or 0.5 ug of Anti-Mouse/Rat CD49e (Integrin alpha 5) Purified (right) followed by Anti-Armenian Hamster IgG FITC. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: eBioHMa5-1 (HMa5-1)
• Applications: FC
• Recommended Dilution: Flow, Functional Assay, IP
• Reactivities: Mouse, Rat
• Host: Hamster
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: integrin alpha 5 (fibronectin receptor alpha)
• Database Link:
  NP_034707
  Entrez Gene 315346 RatEntrez Gene 16402 Mouse
  UniProt ID P11688
• Background: The eBioHMα5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin α5 or VLA-5A. CD49e is a member of the integrin α chain family, and binds with CD29 (integrin β1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The eBioHMα5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMα5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.
• Synonyms: CD49e; FNRA; VLA-5; VLA5A