CD146 (MCAM) Mouse Monoclonal Antibody [Clone ID: P1H12]

CAT#: TA320421

Mouse Anti-CD146 Purified (100 ug)

Product Images

Staining of Human Umbilical Vein Endothelial Cells (HUVEC) with 0.125 ug of Mouse IgG1 K Isotype Control Purified (blue histogram) or 0.125 ug of Anti-CD146 Purified (purple histogram) followed by F (ab')2 Anti-Mouse IgG PE. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: P1H12
• Applications: FC
• Recommended Dilution: Flow, IHC, IP, WB
• Reactivities: Canine, Human, Mouse, Rabbit
• Host: Mouse
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: melanoma cell adhesion molecule
• Database Link:
  NP_006491
  Entrez Gene 84004 MouseEntrez Gene 4162 Human
  UniProt ID P43121
• Background: The monoclonal antibody P1H12 recognizes CD146 also known as MUC18, s-endo, Endo-CAM and Mel-CAM, which is a member of the Ig superfamily of proteins. The expression of CD146 is found on endothelial cells, bone marrow fibroblasts and some tumors (especially melanoma). Recently mesenchymal stromal cells and endometrial stromal cells have also been shown to express CD146. The presence of CD146 on circulating blood cells have been confined to a subset of T cells rather than circulating endothelial cells, as expression of other endothelial markers (CD31 and CD51/61) is negative. Expression can be found on activated lymphocytes. The protein is heavily glycosylated with more than 50% of the mass from carbohydrates.The antibody P1H12 has been reported to crossreact to mouse, rabbit, canine, but not rat.
• Synonyms: CD146; MUC18

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane