Nestin (NES) Mouse Monoclonal Antibody [Clone ID: 10C2]

CAT#: TA320427

Mouse Anti-Human Nestin Purified (25 ug)

Product Images

U251 cell lysates prepared under non-reducing (lane 1) or reducing (lane 2) conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of Anti-Human Nestin Purified. Bands were visualized using Anti-Mouse IgG HRP.

Specifications

Product Data

• Clone Name: 10C2
• Applications: WB
• Recommended Dilution: IHC, WB
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nestin
• Database Link:
  NP_006608
  Entrez Gene 10763 Human
  UniProt ID P48681
• Background: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240 kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system, specifically the neuroepithelium and angiogenic cells, and progenitors of pancreatic islet and mesenchymal cells. Additionally, expression has been identified in a variety of tumors such as glioblastomas and pancreatic tumors. Nestin containing filaments upon differentitation are replaced by cell type specific intermediate filament proteins, such as GFAP. No co-reactivity to rodent nestins has been observed using 10C2 antibody.
• Synonyms: Nbla00170

Reference Data

• Protein Families: ES Cell Differentiation/IPS