TWEAKR (TNFRSF12A) Mouse Monoclonal Antibody [Clone ID: ITEM-4]

CAT#: TA320496

Mouse Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (25 ug)

Product Images

Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 ug of Mouse IgG2a K Isotype Control Purified (open histogram) or 0.5 ug Anti-Human/Mouse CD266 (TWEAK Receptor) Purified (filled histogram) followed byAnti-Mouse IgG FITC. Total viable cells were analyzed.

Specifications

Product Data

• Clone Name: ITEM-4
• Applications: FC
• Recommended Dilution: Flow, IHC, WB
• Reactivities: Human, Mouse
• Host: Mouse
• Clonality: Monoclonal
• Formulation: Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: tumor necrosis factor receptor superfamily member 12A
• Database Link:
  NP_057723
  Entrez Gene 27279 MouseEntrez Gene 51330 Human
  UniProt ID Q9NP84
• Background: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.The ITEM-4 antibody has been show to have blocking function.
• Synonyms: CD266; FN14; TWEAKR

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction