Angiotensinogen (AGT) Rabbit Polyclonal Antibody

CAT#: TA321041

Anti-AGT Rabbit Polyclonal Antibody

Product Images

Predicted band size: 53 kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/1100 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 minutes)

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  Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using AGT antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:2000-5000, WB: 1:1000-5000,IHC: 1:25-100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 34-41 amino acids of human angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53 kDa
• Gene Name: angiotensinogen
• Database Link:
  NP_000020
  Entrez Gene 11606 MouseEntrez Gene 24179 RatEntrez Gene 183 Human
  UniProt ID P01019
• Background: The protein encoded by this gene; pre-angiotensinogen or angiotensinogen precursor; is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product; angiotensin I; is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension; and can cause renal tubular dysgenesis; a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation; and inflammatory bowel disease.
• Synonyms: ANHU; SERPINA8

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Renin-angiotensin system