CUL4A Rabbit Polyclonal Antibody

CAT#: TA321260

Anti-CUL4A Rabbit Polyclonal Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-4: HepG2 Hela 231 cell and Human fetal brain tissue lysates Primary antibody: TA321260 (CUL4A Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 seconds

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA321260 (CUL4A Antibody) at dilution 1/45 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA321260 (CUL4A Antibody) at dilution 1/45, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA321260 (CUL4A Antibody) at dilution 1/45 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA321260 (CUL4A Antibody) at dilution 1/45, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: HepG2, Hela, 231 cell and Human fetal brain tissue lysates
  IHC: 40-200
  Positive control: Human esophagus cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 721-735 amino acids of Human cullin 4A
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88 kDa
• Gene Name: cullin 4A
• Database Link:
  NP_001008895
  Entrez Gene 99375 MouseEntrez Gene 361181 RatEntrez Gene 8451 Human
  UniProt ID Q13619
• Background: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1; DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication.
• Synonyms: cullin 4A; OTTHUMP00000018753; OTTHUMP00000018754

Reference Data

• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis