Aconitase 2 (ACO2) Rabbit Polyclonal Antibody

CAT#: TA321275

Anti-ACO2 rabbit polyclonal antibody

Product Images

Predicted band size: 85 kDa. Positive control: Mouse heart tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds)

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  Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer using ACO2 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification: x 200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:2000-5000, WB: 1:500-2000, IHC: 1:50-200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 766-780 amino acids of human aconitase 2, mitochondrial
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85 kDa
• Gene Name: aconitase 2
• Database Link:
  NP_001089
  Entrez Gene 11429 MouseEntrez Gene 79250 RatEntrez Gene 50 Human
  UniProt ID Q99798
• Background: The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.?
• Synonyms: ACONM; HEL-S-284; ICRD; OCA8; OPA9

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways