Cyclin E1 (CCNE1) Rabbit Polyclonal Antibody

CAT#: TA321312

Anti-CCNE1 (phospho-Thr395) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 47 kDa. Positive control: 293 cells treated with hydroxyurea lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: 293 cells untreated with hydroxyurea lysate Lane 2: 293 cells treated with hydroxyurea lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1000
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47 kDa
• Gene Name: cyclin E1
• Database Link:
  NP_001229
  Entrez Gene 898 Human
  UniProt ID P24864
• Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.
• Synonyms: CCNE; pCCNE1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Prostate cancer, Small cell lung cancer