HSP27 (HSPB1) Rabbit Polyclonal Antibody

CAT#: TA321368

Anti-HSPB1 (Phospho-Ser82) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 23 kDa. Positive control: HL60 cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: HL60 cells untreated with UV lysate Lane 2: HL60 cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

• 

  Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HSPB1 (Phospho-Ser82) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 82 (Q-L-S(p)-S-G) derived from Human HSP27.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23 kDa
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  NP_001531
  Entrez Gene 3315 Human
  UniProt ID P04792
• Background: Involved in stress resistance and actin organization.
• Synonyms: CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; HSP27; HSP28; SRP27

Reference Data

• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway