DUT Rabbit Polyclonal Antibody

CAT#: TA321412

Anti-DUT Rabbit Polyclonal Antibody

Product Images

Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using TA321412 (DUT Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gasrtic cancer tissue using TA321412 (DUT Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA321412 (DUT Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA321412 (DUT Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC: 25-100
  Positive control: Human gasrtic cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 132-244 amino acids of Human Deoxyuridine triphosphatase
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27 kDa
• Gene Name: deoxyuridine triphosphatase
• Database Link:
  NP_001939
  Entrez Gene 110074 MouseEntrez Gene 497778 RatEntrez Gene 1854 Human
  UniProt ID P33316
• Background: This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous; homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication; and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA; which induces extensive excision repair mediated by uracil glycosylase. This repair process; resulting in the removal and reincorporation of dUTP; is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
• Synonyms: dUTPase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism