hnRNP K (HNRNPK) Rabbit Polyclonal Antibody

CAT#: TA321440

Anti-HNRNPK Rabbit Polyclonal Antibody

Product Images

Predicted band size: 51 kDa. Positive control: HeLa cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-2000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to N terminal 280 amino acids of human heterogeneous nuclear ribonucleoprotein K
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51 kDa
• Gene Name: heterogeneous nuclear ribonucleoprotein K
• Database Link:
  NP_002131
  Entrez Gene 15387 MouseEntrez Gene 117282 RatEntrez Gene 3190 Human
  UniProt ID P61978
• Background: Heterogeneous nuclear ribonucleoprotein K (hnRNP K) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm. hnRNP K contains three unique structural motifs termed KH domains that bind poly(C) DNA and RNA sequences. Intricate architecture enables hnRNP K to facilitate mRNA biosynthesis, transcriptional regulation, and signal transduction. Research studies have shown that cytoplasmic hnRNP K expression is increased in oral squamous cell carcinoma and pancreatic cancer, and may be a potential prognostic factor. hnRNP K coordinates with p53 to regulate its target gene transcription in response to DNA damage. Proteasome degradation of hnRNP K is mediated by E3 ligase MDM2. The interaction between hnRNP K and c-Src leads to hnRNP K phosphorylation, which allows for hnRNP K activation of silenced mRNA translation.
• Synonyms: CSBP; HNRPK; TUNP

Reference Data

• Protein Pathways: Spliceosome