Vimentin (VIM) Rabbit Polyclonal Antibody

CAT#: TA321578

Anti-VIM Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: Hela cell lysate Primary antibody: TA321578 (VIM Antibody) at dilution 1/4000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 3 second

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  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA321578 (VIM Antibody) at dilution 1/60. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA321578 (VIM Antibody) at dilution 1/60. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 2000-5000
  WB positive control: Hela cell lysate
  IHC: 50-300
  Positive control: Human tonsil
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 96-407 amino acids of Human Vimentin
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: vimentin
• Database Link:
  NP_003371
  Entrez Gene 22352 MouseEntrez Gene 81818 RatEntrez Gene 7431 Human
  UniProt ID P08670
• Background: This gene encodes a member of the intermediate filament family. Intermediate filamentents; along with microtubules and actin microfilaments; make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape; integrity of the cytoplasm; and stabilizing cytoskeletal interactions. It is also involved in the immune response; and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment; migration; and cell signaling. Mutations in this gene causes a dominant; pulverulent cataract.
• Synonyms: CTRCT30; HEL113

Reference Data

• Protein Families: ES Cell Differentiation/IPS