EIF4EBP1 Rabbit Polyclonal Antibody

CAT#: TA321619

Anti-EIF4EBP1 rabbit polyclonal antibody

Product Images

Predicted band size: 13kDa. Positive control: 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 8+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/530 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm and Nucleus. Positive control: Human colon cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using EIF4EBP1 antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:2000-5000, WB: 1:200-1000,IHC: 1:50-200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 4-118 amino acids of human eukaryotic translation initiation factor 4E binding protein 1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13 kDa
• Gene Name: eukaryotic translation initiation factor 4E binding protein 1
• Database Link:
  NP_004086
  Entrez Gene 13685 MouseEntrez Gene 116636 RatEntrez Gene 1978 Human
  UniProt ID Q13541
• Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
• Synonyms: 4E-BP1; 4EBP1; BP-1; PHAS-I

Reference Data

• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Insulin signaling pathway, mTOR signaling pathway