Calreticulin (CALR) Rabbit Polyclonal Antibody

CAT#: TA321647

Anti-CALR Rabbit Polyclonal Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-3: Hela cells 293T cells NIH/3T3 cells Primary antibody: TA321647 (CALR Antibody) at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA321647 (CALR Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA321647 (CALR Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA321647 (CALR Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human gastric cancer tissue using TA321647 (CALR Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Hela, 293T and NIH/3T3 cells
  IHC: 50-200
  Positive control: Human ovarian cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 20-218 amino acids of human calreticulin
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48 kDa
• Gene Name: calreticulin
• Database Link:
  NP_004334
  Entrez Gene 12317 MouseEntrez Gene 64202 RatEntrez Gene 811 Human
  UniProt ID P27797
• Background: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus; suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR; which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies; it is highly conserved among species; and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element.
• Synonyms: cC1qR; CRT; HEL-S-99n; RO; SSA

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors
• Protein Pathways: Antigen processing and presentation