ASK1 (MAP3K5) Rabbit Polyclonal Antibody

CAT#: TA321734

Anti-MAP3K5 Rabbit Polyclonal Antibody

Product Images

Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA321734 (MAP3K5 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA321734 (MAP3K5 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA321734 (MAP3K5 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA321734 (MAP3K5 Antibody) at dilution 1/20, treated with synthetic peptide. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: IHC: 25-100
  Positive control: Human colon cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 1-14 amino acids of Human mitogen-activated protein kinase kinase kinase 5
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mitogen-activated protein kinase kinase kinase 5
• Database Link:
  NP_005914
  Entrez Gene 26408 MouseEntrez Gene 365057 RatEntrez Gene 4217 Human
  UniProt ID Q99683
• Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK); MAPK kinase (MKK or MEK); and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase; MAPK kinase/MEK; which in turn activates MAPK. The kinases of these signaling cascades are highly conserved; and homologs exist in yeast; Drosophila; and mammalian cells. MAPKKK5 contains 1;374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1; MAPKK4) in vitro; and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
• Synonyms: ASK1; MAPKKK5; MEKK5

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway