NIT2 Rabbit Polyclonal Antibody

CAT#: TA321872

Anti-NIT2 Rabbit Polyclonal Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-3: Mouse liver tissue Mouse kidney tissue RAW264.7 cells Primary antibody: TA321872 (NIT2 Antibody) at dilution 1/950 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 90 seconds

• 

  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA321872 (NIT2 Antibody) at dilution 1/40 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA321872 (NIT2 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA321872 (NIT2 Antibody) at dilution 1/40 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA321872 (NIT2 Antibody) at dilution 1/40, treated with synthetic peptide. (Original magnification: ×200)

Show all 5 images

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Mouse liver and kidney tissue, RAW264.7 cells
  IHC: 50-200
  Positive control: Human colon cancer
  Predicted cell location: Cytoplasm, Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 264-276 amino acids of Human itrilase family, member 2
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31 kDa
• Gene Name: nitrilase family member 2
• Database Link:
  NP_064587
  Entrez Gene 52633 MouseEntrez Gene 288174 RatEntrez Gene 56954 Human
  UniProt ID Q9NQR4
• Background: Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate; respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
• Synonyms: HEL-S-8a