JNK3 (MAPK10) Rabbit Polyclonal Antibody

CAT#: TA321931

Anti-MAPK10 Rabbit Polyclonal Antibody

Product Images

Predicted band size: 53 kDa. Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 minutes)

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  Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/5-20 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using MAPK10 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:1000-5000, WB: 1:500-2000, IHC: 1:5-20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region derived from 416-428 amino acids of Human mitogen-activated protein kinase 10
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32 kDa
• Gene Name: mitogen-activated protein kinase 10
• Database Link:
  NP_620448
  Entrez Gene 25272 RatEntrez Gene 26414 MouseEntrez Gene 5602 Human
  UniProt ID P53779
• Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals; and are involved in a wide variety of cellular processes such as proliferation; differentiation; transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization; this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2; a receptor-regulated MAP kinase scaffold protein; is found to interact with; and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate; and inhibit the activity of this kinase; which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
• Synonyms: JNK3; JNK3A; p54bSAPK; p493F12; PRKM10; SAPK1b

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway