ACAT1 (ACACA) Rabbit Polyclonal Antibody

CAT#: TA321968

Anti-ACACA (Phospho-Ser79) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 266 kDa. Positive control: 293 cells treated with AICAR lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane 1: 293 cells untreated with AICAR lysate Lane 2: 293 cells treated with AICAR lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500-1000
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of serine 79(S-M-S(p)-G-L) derived from Human Acetyl-CoA Carboxylase.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 266 kDa
• Gene Name: acetyl-CoA carboxylase alpha
• Database Link:
  NP_942132
  Entrez Gene 31 Human
  UniProt ID Q13085
• Background: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
• Synonyms: ACAC; ACC; ACC1; ACCA

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism