ACAD11 Rabbit Polyclonal Antibody

CAT#: TA322146

Anti-ACAD11 rabbit polyclonal antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: Mouse kidney tissue Mouse heart tissue lysates Primary antibody: TA322146 (ACAD11 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA322146 (ACAD11 Antibody) at dilution 1/55 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA322146 (ACAD11 Antibody) at dilution 1/55, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Mouse kidney tissue, Mouse heart tissue lysates
  IHC: 50-200
  Positive control: Human esophagus cancer
  Predicted cell location: Cytoplasm and Cell membrane
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 121-400 amino acids of human acyl-CoA dehydrogenase family, member 11
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87 kDa
• Gene Name: acyl-CoA dehydrogenase family member 11
• Database Link:
  NP_115545
  Entrez Gene 102632 MouseEntrez Gene 315973 RatEntrez Gene 84129 Human
  UniProt ID Q709F0
• Background: The deduced full-length?ACAD11?protein contains an N-terminal aminoglycoside phosphotransferase domain, followed by a mitochondria localization signal and ACAD N-terminal, middle, and C-terminal domains. It also has a glycosylation site and a C-terminal peroxisome-targeting signal. The ACAD domain contains a catalytic aspartate rather than the catalytic glutamate found in most other ACADs. Real-time RT-PCR detected variable?ACAD11?expression in all tissues examined, with highest expression in adult brain, followed by heart, liver, spinal cord, and kidney. Western blot analysis of fractionated human cerebellum and kidney revealed thatACAD11?associated with mitochondrial membranes, but not mitochondrial matrix. Immunohistochemical analysis showed that?ACAD11colocalized with a mitochondrial marker in human neuroblastoma cells.
• Synonyms: ACAD-11

Reference Data

• Protein Families: Druggable Genome