Cyclin B1 (CCNB1) Rabbit Polyclonal Antibody

CAT#: TA322149

Anti-CCNB1 (phospho-Ser147) Rabbit Polyclonal Antibody

Product Images

Predicted band size: 60 kDa. Positive control: MDA cells untreated or treated with Anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane 1: MDA cells untreated with Anisomycin lysate Lane 2: MDA cells treated with Anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using CCNB1 (phospho-Ser147) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)

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  Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using CCNB1 (phospho-Ser147) antibody at dilution 1/100. (Original magnification: ×200)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
• Reactivities: Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Serine 147 (A-F-S(p)-D-V) derived from Human Cyclin B1.
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60 kDa
• Gene Name: cyclin B1
• Database Link:
  NP_114172
  Entrez Gene 891 Human
  UniProt ID P14635
• Background: The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
• Synonyms: CCNB

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, p53 signaling pathway, Progesterone-mediated oocyte maturation