ATPase Inhibitory Factor 1 (ATPIF1) Rabbit Polyclonal Antibody

CAT#: TA322326

Anti-ATPIF1 Rabbit Polyclonal Antibody

Product Images

Gel: 12%SDS-PAGE Lysate: 40 μg Lane: K562 cell lysate Primary antibody: TA322326 (ATP5IF1 Antibody) at dilution 1/550 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 90 seconds

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA322326 (ATP5IF1 Antibody) at dilution 1/85 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using TA322326 (ATP5IF1 Antibody) at dilution 1/85, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: K562 cell lysate
  IHC: 50-200
  Positive control: Human colorectal cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 26-106 amino acids of human ATPase inhibitory factor 1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12 kDa
• Gene Name: ATPase inhibitory factor 1
• Database Link:
  NP_057395
  Entrez Gene 11983 MouseEntrez Gene 25392 RatEntrez Gene 93974 Human
  UniProt ID Q9UII2
• Background: ATPase inhibitor; mitochondrial?is an?enzyme?that in humans is encoded by the?ATPIF1?gene. This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. It prevents ATPase from switching to ATP hydrolysis during collapse of the electrochemical gradient; for example during oxygen deprivation ATP synthase inhibitor forms a one to one complex with the F1 ATPase; possibly by binding at the alpha-beta interface. It is thought to inhibit ATP synthesis by preventing the release of ATP. The inhibitor has two oligomeric states; dimer (the active state) and tetramer.
• Synonyms: ATPI; ATPIP; IP