IGSF6 Rabbit Polyclonal Antibody

CAT#: TA322553

Anti-IGSF6 Rabbit Polyclonal Antibody

Product Images

Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA322553 (IGSF6 Antibody) at dilution 1/10 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA322553 (IGSF6 Antibody) at dilution 1/10, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: IHC: 5-20
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a region derived from 28-153 amino acids of human immunoglobulin superfamily, member 6
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: immunoglobulin superfamily member 6
• Database Link:
  NP_005840
  Entrez Gene 80719 MouseEntrez Gene 171064 RatEntrez Gene 10261 Human
  UniProt ID O95976
• Background: IGSF6 (DORA); a novel member of the immunoglobulin superfamily (IGSF) from human and rat expressed in dendritic and myeloid cells. Using a probe from the open reading frame of the rat cDNA; we isolated a cosmid which contains the entire mouse gene. By comparative analysis and reverse transcriptase polymerase chain reaction; we defined the intron/exon structure and the mRNA of the mouse gene and; with respect to human BAC clones; the human gene. The genes span 10 kb (mouse) and 12 kb (human); with six exons arranged in a manner similar to other members of the IGSF. All intron/exon boundaries follow the GT-AG rule. Expression of the mouse Igsf6 gene is restricted to cells of the immune system; particularly macrophages. Northern blot revealed a single mRNA of 2.5 kb; in contrast to the human gene which is expressed as two mRNAs of 1 and 2.5 kb. The human and mouse genes were localized to a locus associated with inflammatory bowel disease. Analysis of the flanking regions of the Igsf6 gene revealed the presence of an unrelated gene; transcribed from the opposite strand of the DNA and oriented such that the Igsf6 gene is encoded entirely within an intron. An identical organization is seen in human.
• Synonyms: DORA

Reference Data

• Protein Families: Transmembrane