Carbonic Anhydrase III (CA3) Rabbit Polyclonal Antibody

CAT#: TA322662

Anti-CA3 Rabbit Polyclonal Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-3: Rat skeletal muscle tissue Mouse skeletal muscle tissue Human liver tissue lysates Primary antibody: TA322662 (CA3 Antibody) at dilution 1/800 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 30 seconds

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA322662 (CA3 Antibody) at dilution 1/120 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human breast cancer tissue using TA322662 (CA3 Antibody) at dilution 1/120, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Rat skeletal muscle tissue, Mouse skeletal muscle tissue, Human liver tissue lysates
  IHC: 100-200
  Positive control: Human breast cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to N terminal 260 amino acids of Human Carbonic anhydrase 3
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30 kDa
• Gene Name: carbonic anhydrase 3
• Database Link:
  NP_005172
  Entrez Gene 761 Human
  UniProt ID P07451
• Background: Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.
• Synonyms: CAIII; Car3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism